用于检测施马伦贝格病毒的套式rt-pcr引物组及试剂盒

Nested-set RT-PCR primer set and kit for detecting Schmallenberg virus

Abstract

本发明公开了一种用于检测施马伦贝格病毒的套式RT-PCR引物组及试剂盒,所述引物组的序列如SEQIDNO.1~4所示,所述试剂盒中含有该引物组及RNA提取试剂、反转录试剂、施马伦贝格病毒阳性对照和阴性对照。本发明建立的试剂盒具有特异、敏感、重复性好、快速、费用低廉等优点,可用于施马伦贝格病毒的快速初筛。
The invention discloses a nested-set RT-PCR primer set and a kit for detecting Schmallenberg virus. The sequence of the primer set is shown by SEQIDNO.1-4, and the kit contains the primer set, an RNA (ribonucleic acid) extracting reagent, a reverse transcription reagent, a Schmallenberg virus positive control and a Schmallenberg virus negative control. The kit disclosed by the invention has the advantages of specificity, sensitivity, good repeatability, fastness, low cost and the like, and can be used for quick preliminary screening of the Schmallenberg virus.

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