一种疏风解毒胶囊的质量控制方法

Quality control method for capsules with functions of dispelling wind and removing toxicity

Abstract

The invention provides a detection method for capsules with functions of dispelling wind and removing toxicity. The detection method is used for detecting hastatoside, verbenalin, polydatin, forsythiaside A, verbascoside, glabrolide monoammonium salt and rheum emodin in the capsules simultaneously with a high-efficiency liquid chromatography, wherein the high-efficiency liquid chromatography is performed under following conditions: a chromatographic column adopts octadecyl-silane-bonded silica gel as a filling material; mobile phases comprise a mobile phase A which is acetonitrile and a mobile phase B which is an acidic aqueous solution, and gradient elution is carried out; the flow velocity is 1.0ml/min; the column temperature is 30 DEG C; the detection wavelength is 210nm-400nm; and the number of theoretical plates is calculated according to rheum emodin peaks, and is not less than 5000. According to the detection method provided by the invention, the content of seven active components such as hastatoside, verbenalin, polydatin, forsythiaside A, verbascoside, glabrolide monoammonium salt and rheum emodin in the capsules can be detected simultaneously, so that the active components of the capsules with functions of dispelling wind and removing toxicity can be comprehensively represented. The detection method has the characteristics of high precision, stability and repeatability.
本发明提供一种疏风解毒胶囊的检测方法,采用高效液相色谱法同时检测疏风解毒胶囊中的戟马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酯单铵盐、大黄素,其中,高效液相色谱法的条件包括:色谱柱为以十八烷基硅烷键合硅胶为填充材料;流动相包括:流动相A为乙腈,流动相B为酸性水溶液,进行梯度洗脱;流速:1.0ml/min;柱温:30℃;检测波长:210nm~400nm;理论塔板数按大黄素峰计算,应不低于5000。本发明的检测方法,可以同时检测疏风解毒胶囊中戟马鞭草苷、马鞭草苷、虎杖苷、连翘酯苷A、毛蕊花糖苷、甘草酯单铵盐、大黄素这7种有效成分的含量,从而能够较为全面地表征疏风解毒胶囊活性组分,具有精密度、稳定性、重复性高的特点。

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    CN-107233511-AOctober 10, 2017安徽济人药业有限公司一种疏风解毒胶囊及其制备方法与检测方法及用途